Soil N2O flux and nitrification and denitrification gene responses to feed-induced differences in the composition of dairy cow faeces

Abstract Faeces from cows with different milk yield and non-lactating were applied to soil investigate whether N 2 O efflux is related feeding-induced differences in faecal microbiome abundances of nitrification denitrification genes. Fungal 18S-rRNA gene abundance was the highest faeces group. The 18S-rRNA/ergosterol ratio showed a strong positive correlation 18S-rRNA/fungal glucosamine ratio. milk-yield groups did not affect bacterial 16S rRNA, AOB amoA , nirS nos Z clade I, or 16S-rRNA/muramic acid (MurN) In contrast, nir K generally lowest high-yield 16S-rRNA/MurN 16S-rRNA/bacterial PLFA Cow application increased microbial biomass ergosterol contents as well compared non-amended control soil. Cumulative ?CO roughly twice high control, without between treatments. ?N 16-fold increase after applying cow soil, which above sevenfold treatment. positively MurN total concentration but also nirK at day 14. comparison genome markers cell wall (glucosamine) membrane components (ergosterol) that fungal cells much larger energy-rich than C-limited A diet reduced protein decreased amended